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Gene Synthesis> | Flag-Zasp52-RR (FlyBase ID: FBtr0329912), FSH-Zasp52-RK (FlyBase ID: FBtr0302163) and FSH-Zasp52-LIM234were synthesized by GenScript and cloned into pUAST-attb (for RR, GenBank: EF362409)..., Zasp52-PDZ, Zasp52-ZM, Zasp52-LIM1, Zasp52-STOP155, Zasp52-STOP143, and Zasp52-STOP112 were synthesized or generated by site-directed mutagenesis by GenScript and cloned into pGEX-5X-1 to generate GST.... | Get A Quote |
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Z-discs are organizing centers that establish and maintain myofibril structure and function. Important Z-disc proteins are α-actinin, which cross-links actin thin filaments at the Z-disc and Zasp PDZ domain proteins, which directly interact with α-actinin. Here we investigate the biochemical and genetic nature of this interaction in more detail. Zasp52 is the major Drosophila Zasp PDZ domain protein, and is required for myofibril assembly and maintenance. We show by in vitro biochemistry that the PDZ domain plus a C-terminal extension is the only area of Zasp52 involved in the interaction with α-actinin. In addition, site-directed mutagenesis of 5 amino acid residues in the N-terminal part of the PDZ domain,... More