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Sensitive detection of microRNA in complex biological samples by using two stages DSN-assisted target recycling signal amplification method

Biosens Bioelectron. 2017-01; 
ZhangKai, WangKe, ZhuXue, XuFei, XieMi
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PCR and Cloning … (St. Louis, Missouri, USA). The strand sequences were purchased from Genscript Biotech. Co., Ltd. (Nanjing, China) with the sequences as shown in Table 1. Duplex-specific nuclease (DSN) was purchased from Newbornco Co., Ltd (Shenzhen, China) … Get A Quote

摘要

MicroRNA (miRNA) has become an important biomarker candidate for cancer diagnosis, prognosis, and therapy. In this study, we have developed a novel fluorescence method for sensitive and specific miRNA detection via duplex specific nuclease (DSN) signal amplification and demonstrated its practical application in biological samples. Malachite green (MG) was employed as a "label-free" signal transducer since fluorescence of MG could be enhanced by 100-fold when MG were binding to a G-quadruplex structure formed within the d(GT)G sequence. The proposed signal amplification strategy is an integrated "biological circuit" designed to initiate a cascade of enzymatic reactions in order to detect, amplify, and ... More

关键词

Amplification method,Cell lysates,DSN,Malachite green,M