| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | … After transformation of XL1 Blue cells, three clones from the samples of circularized HPV11 DNA, HPV11.HaCaT (P3 and P8) and A3A-HPV11.HaCaT cells were picked and sequenced. Sequencing analysis was performed by Genscript Corporation (Nanjing, China). Table 1 … | Get A Quote |
Condyloma acuminatum， infected by low-risk human papillomaviruses (e.g.， HPV6 and HPV11)， is one of the most widespread sexually transmitted diseases. Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 proteins (APOBEC3s， A3s) are cellular cytidine deaminases acting as antiviral factors through hypermutation of viral genome. However， it remains unknown whether A3s results in HPV11 gene mutations and interferon-ω (IFN-ω) exhibits antiviral activities through the A3s system. Here we investigated whether enhanced APOBEC3A (A3A) resulted in the E6 gene mutations and explore the effects of recombinant human interferon-ω (rhIFN-ω) on A3s/E6 expression in HaCaT keratinocytes containing the... More
