The bacterial membrane represents an attractive target for the design of new antibiotics to combat widespread bacterial resistance. Understanding how antimicrobial peptides (AMPs) and other membrane-active agents attack membranes could facilitate the design of new， effective antimicrobials. Despite intense study of AMPs on model membranes， we do not know how well the mechanism of attack translates to real biological membranes. To that end， we have characterized the attack of AMPs on Escherichia coli cytoplasmic membranes and directly compared this action to model membranes. AMPs induce membrane permeability in E. coli spheroplasts or giant unilamellar vesicles (GUVs) under well-defined concentrations of ... More
The bacterial membrane represents an attractive target for the design of new antibiotics to combat widespread bacterial resistance. Understanding how antimicrobial peptides (AMPs) and other membrane-active agents attack membranes could facilitate the design of new， effective antimicrobials. Despite intense study of AMPs on model membranes， we do not know how well the mechanism of attack translates to real biological membranes. To that end， we have characterized the attack of AMPs on Escherichia coli cytoplasmic membranes and directly compared this action to model membranes. AMPs induce membrane permeability in E. coli spheroplasts or giant unilamellar vesicles (GUVs) under well-defined concentrations of AMPs and fluorescent molecules. The action of AMPs on spheroplasts is unique in producing an intracellular fluorescence intensity time curve that increases in a sigmoidal fashion to a steady state. This regular pattern is reproducible by melittin， LL37， and alamethicin but not by CCCP or daptomycin， agents known to cause ion leakage. Remarkably， a similar pattern was also reproduced in GUVs. Indeed the steady-state membrane permeability induced by AMPs is quantitatively the same in spheroplasts and GUVs. There are， however， interesting dissimilarities in details that reveal differences between bacterial and lipid membranes. Spheroplast membranes are permeabilized by a wide range of AMP concentrations to the same steady-state membrane permeability. In contrast， only a narrow range of AMP concentrations permeabilized GUVs to a steady state. Tension in GUVs also influences the action of AMPs， whereas the spheroplast membranes are tensionless. Despite these differences， our results provide a strong support for using model membranes to study the molecular interactions of AMPs with bacterial membranes. As far as we know， this is the first time the actions of AMPs， on bacterial membranes and on model membranes， have been directly and quantitatively compared.
