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Mutagenesis Services> | … residues. Mutations in GluD2-LBD were introduced using overlap extension PCR 28 , except for the GluD2-LBD-(H)GluN1 mutant, which was produced by GenScript USA Inc. (Piscataway, NJ, USA). GluN1-LBD construct. The … | Get A Quote |
Ionotropic glutamate receptors (iGluRs) are responsible for most of the fast excitatory communication between neurons in our brain. The GluD2 receptor is a puzzling member of the iGluR family: It is involved in synaptic plasticity, plays a role in human diseases, e.g. ataxia, binds glycine and D-serine with low affinity, yet no ligand has been discovered so far that can activate its ion channel. In this study, we show that the hinge region connecting the two subdomains of the GluD2 ligand-binding domain is responsible for the low affinity of D-serine, by analysing GluD2 mutants with electrophysiology, isothermal titration calorimetry and molecular dynamics calculations. The hinge region is highly ... More