至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

High sensitive single chain variable fragment screening from a microcystin-LR immunized mouse phage antibody library and its application in immunoassay.

Talanta. 2019-05; 
XuChongxin, LiuXiaoqin, LiuYuan, ZhangXiao, ZhangCunzheng, LiJianhong, LiuXia
Products/Services Used Details Operation
Monoclonal Antibody Services … antibody and anti-His-HRP monoclonal antibody were purchased from GenScript Bio … systems, DNA marker and protein marker standards were purchased from Com Win Biotech … electrophoresis analysis on 1% agarose gels, and sequenced by Invitrogen Biotechnology Co., Ltd … Get A Quote

摘要

Microcystin-LR (MC-LR) is one of common high-toxic biotoxins produced by cyanobacteria in waterbody. A high sensitive and convenient detection method is necessary for monitoring for MC-LR. To establish a high sensitive indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) based on single chain variable fragment (scFv) for detecting MC-LR, 16 positive anti-MC-LR phage scFv particles were screened out from a MC-LR-immunized mouse phage scFv library, which was successfully constructed with the capacity of 8.67 × 10 CFU/mL. The most positive anti-MC-LR phage scFv (MscFv7) was successfully expressed in Escherichia coli (E.coli) HB2151. The molecular weight (M.W.) of expressed protein was about 3... More

关键词

Immunoassay,Microcystin-LR,Phage antibody library,Single chain variable frag