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Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies.

BMC Vet. Res.. 2019-03; 
ZhangSujiao, WangDongliang, JiangYifan, LiZhoumian, ZouYawen, LiMeng, YuHaoyang, HuangKun, YangYi, WangNai
Products/Services Used Details Operation
Gene Synthesis A PCV3 cap gene (GenBank accession number. AQZ26221) was optimized and synthesized by GenScript Company (Nanjing, China). Get A Quote
Bacterial Expression System Get A Quote

摘要

Porcine circovirus type 3 (PCV3), recently widely isolated from pigs with various clinical conditions, is likely globally epidemic. However, development of serological diagnosis for PCV3 in pigs is ongoing. Our objectives were to: 1) establish an indirect ELISA, using PCV3 capsid protein (Cap) prepared by Baculovirus Expression Vector System (BEVS) as a high-quality coating antigen for detection of PCV3-associated antibodies in serum samples; and 2) use this ELISA to conduct a serological survey for PCV3 in various regions of Hunan province, China.

关键词

Diagnostic,ELISA,Pig,Porcine circovirus ty