至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Synthesis of a non-natural glucose-2-phosphate ester able to dupe the acc system of Agrobacterium fabrum.

Org. Biomol. Chem.. 2019-01; 
LiSi-Zhe, VigourouxArmelle, AhmarMohammed, El SahiliAbbas, SoulèreLaurent, SagoLaïla, CornuDavid, MoréraSolange, Queneau
Products/Services Used Details Operation
PCR and Cloning … optimization for the expression in E. coli and inserted into pET-21b plasmid using NdeI and BamHI restriction enzyme (Genscript, Piscataway, NJ) … A PhD grant to SZL from the China Scholarship Council is gratefully acknowledged, as well as financial support from CNRS and … Get A Quote

摘要

The first non-natural derivative of the rare d-glucose-2-phosphate (G2P), namely glucose-2-(O-lactic acid phosphate) (G2LP), has been synthesized. When used as sole carbon source, G2LP enables bacterial growth of the plant pathogenic strain Agrobacterium fabrum C58 (formerly referred to as Agrobacterium tumefaciens). X-ray crystallography and affinity measurements investigations reveal that G2LP binds the periplasmic binding protein (PBP) AccA similarly to the natural compounds and with the same affinity. Moreover, enzymatic assays show that it is able to serve as substrate of the phosphodiesterase AccF. The properties found for G2LP demonstrate that the very unusual glucose-2-phosphoryl residue, pres... More

关键词