Products/Services Used | Details | Operation |
---|---|---|
PCR and Cloning> | … optimization for the expression in E. coli and inserted into pET-21b plasmid using NdeI and BamHI restriction enzyme (Genscript, Piscataway, NJ) … A PhD grant to SZL from the China Scholarship Council is gratefully acknowledged, as well as financial support from CNRS and … | Get A Quote |
The first non-natural derivative of the rare d-glucose-2-phosphate (G2P), namely glucose-2-(O-lactic acid phosphate) (G2LP), has been synthesized. When used as sole carbon source, G2LP enables bacterial growth of the plant pathogenic strain Agrobacterium fabrum C58 (formerly referred to as Agrobacterium tumefaciens). X-ray crystallography and affinity measurements investigations reveal that G2LP binds the periplasmic binding protein (PBP) AccA similarly to the natural compounds and with the same affinity. Moreover, enzymatic assays show that it is able to serve as substrate of the phosphodiesterase AccF. The properties found for G2LP demonstrate that the very unusual glucose-2-phosphoryl residue, pres... More