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Recognition by host nuclear transport proteins drives disorder-to-order transition in Hendra virus V

Sci Rep.. 2018-01; 
Atkinson SC, Audsley MD, Lieu KG, Marsh GA, Thomas DR, Heaton SM, Paxman JJ, Wagstaff KM, Buckle AM, Moseley GW, Jans DA, Borg NA.
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Gene Synthesis Te full-length HeV V gene (GenBank accession no. NC001906) from a HeV horse isolate (isolate Horse/Australia/Hendra/1994)53 was synthesized by GenScript® and provided in plasmid pUC57... Get A Quote
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摘要

Hendra virus (HeV) is a paramyxovirus that causes lethal disease in humans, for which no vaccine or antiviral agent is available. HeV V protein is central to pathogenesis through its ability to interact with cytoplasmic host proteins, playing key antiviral roles. Here we use immunoprecipitation, siRNA knockdown and confocal laser scanning microscopy to show that HeV V shuttles to and from the nucleus through specific host nuclear transporters. Spectroscopic and small angle X-ray scattering studies reveal HeV V undergoes a disorder-to-order transition upon binding to either importin α/β1 or exportin-1/Ran-GTP, dependent on the V N-terminus. Importantly, we show that specific inhibitors of nuclear transport pre... More

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