Products/Services Used | Details | Operation |
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Gene Synthesis> | Te full-length HeV V gene (GenBank accession no. NC001906) from a HeV horse isolate (isolate Horse/Australia/Hendra/1994)53 was synthesized by GenScript® and provided in plasmid pUC57... | Get A Quote |
Bacterial Expression System> | Get A Quote |
Hendra virus (HeV) is a paramyxovirus that causes lethal disease in humans, for which no vaccine or antiviral agent is available. HeV V protein is central to pathogenesis through its ability to interact with cytoplasmic host proteins, playing key antiviral roles. Here we use immunoprecipitation, siRNA knockdown and confocal laser scanning microscopy to show that HeV V shuttles to and from the nucleus through specific host nuclear transporters. Spectroscopic and small angle X-ray scattering studies reveal HeV V undergoes a disorder-to-order transition upon binding to either importin α/β1 or exportin-1/Ran-GTP, dependent on the V N-terminus. Importantly, we show that specific inhibitors of nuclear transport pre... More