| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | Two recombinant clones of each sample were selected and sequenced using BigDye Terminator Mix (Genscript, Nanjing, China). | Get A Quote |
The apicomplexan parasite Theileria orientalis is a tick-borne intracellular protozoan parasite that is widely distributed throughout China. It causes bovine theileriosis in infected cattle， which results in huge economic losses to the cattle industry. In this study， the infection status of T. orientalis was determined in 260 blood samples from cattle from seven provinces across China. Results of a major piroplasm surface protein (MPSP)-PCR assay revealed that an average of 36.5% (95/260) of cattle was positive for T. orientalis infection. Based on the MPSP gene sequences， phylogenetic analysis revealed that these isolates of T. orientalis comprised of eight MPSP types， 1， 2， 3， 4， 5， 7， N1... More
