De novo design of RNA-binding proteins with a prion-like domain related to ALS/FTD proteinopathies
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PCR Cloning and Subcloning> |
cDNAs encoding SYG/SYGQ-NES/NLS and synthetic prion-forming domain (sPFD)/ control prion-forming domain (cPFD)-NES/NLS (Sequences are shown in Supplementary information) were synthesized (GenScript, NJ, USA) and subcloned into pcDNA (3.1) or pEGFP-N1 (Clontech, CA, USA) or. Wild type and mutant TDP-43 and FUS were described in previous paper |
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