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De novo design of RNA-binding proteins with a prion-like domain related to ALS/FTD proteinopathies

Scientific REPORTS. 2017; 
Kana Mitsuhashi, Daisuke Ito, Kyoko Mashima, Munenori Oyama, Shinichi Takahashi & Norihiro Suzuki
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PCR Cloning and Subcloning cDNAs encoding SYG/SYGQ-NES/NLS and synthetic prion-forming domain (sPFD)/ control prion-forming domain (cPFD)-NES/NLS (Sequences are shown in Supplementary information) were synthesized (GenScript, NJ, USA) and subcloned into pcDNA (3.1) or pEGFP-N1 (Clontech, CA, USA) or. Wild type and mutant TDP-43 and FUS were described in previous paper Get A Quote

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