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Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation

Cold Spring Harbor Laboratory Press. 2017; 
Hengshan Zhang and Maria A. Schumacher
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Gene Synthesis A gene encoding the designed MBP–ParA construct codon-optimized for E. coli expression was purchased from Genscript and cloned into the pET15b vector using NdeI and BamHI sites, which also generated a his tag N-terminal to MBP. Get A Quote

摘要

关键词

ParA; Walker-box; DNA segregation; nucleoid; HDR; ParB; diffusion ratchet
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