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Prc1E and Kif4A control microtubule organization within and between large Xenopus egg asters

Mol Biol Cell. 2018-02; 
Nguyen PA,, Field CM,, Mitchison TJ
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Bacterial Expression System The X. laevis Prc1E coding sequence (codon optimized for Escherichia coli and insect cell expression; GenScript) was subcloned into pET-28b bacterial Get A Quote

摘要

The cleavage furrow in Xenopus zygotes is positioned by two large microtubule asters that grow out from the poles of the first mitotic spindle. Where these asters meet at the midplane, they assemble a disk-shaped interaction zone consisting of anti-parallel microtubule bundles coated with chromosome passenger complex (CPC) and centralspindlin that instructs the cleavage furrow. Here we investigate the mechanism that keeps the two asters separate and forms a distinct boundary between them, focusing on the conserved cytokinesis midzone proteins Prc1 and Kif4A. Prc1E, the egg orthologue of Prc1, and Kif4A were recruited to anti-parallel bundles at interaction zones between asters in Xenopus egg extracts. Prc1E was... More

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