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Vancomycin does not affect the enzymatic activities of purified VanSA

PLoS ONE. 2019-06; 
Upton EC, Maciunas LJ, Loll PJ
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Bacterial Expression System A gene encoding full-length VanSA (Uniprot Q06240) was synthesized using codon optimization for E. coli expression (Genscript) and cloned into the in-house vector pETCH Get A Quote

摘要

VanS is a membrane-bound sensor histidine kinase responsible for sensing vancomycin and activating transcription of vancomycin-resistance genes. In the presence of vancomycin, VanS phosphorylates the transcription factor VanR, converting it to its transcriptionally active form. In the absence of vancomycin, VanS dephosphorylates VanR, thereby maintaining it in a transcriptionally inactive state. To date, the mechanistic details of how vancomycin modulates VanS activity have remained elusive. We have therefore studied these details in an in vitro system, using the full-length VanS and VanR proteins responsible for type-A vancomycin resistance in enterococci. Both detergent- and amphipol-solubilized VanSA display... More

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