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Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice (Oryza sativa L.) at the seedling stage

3 Biotech.. 2019-07; 
Nawaz G#, Han Y#, Usman B, Liu F, Qin B, Li R
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Common Tools … Specific primers (PRP1-F and PRP1-R) for OsPRP1 and (SODF/SODR, PODF/PODR, and CATF/CATR) were designed for antioxidant genes using online GenScript website tool (https://www.genscript.com/) (Additional file … Get A Quote

摘要

Proline-rich proteins (PRPs) play multiple physiological and biochemical roles in plant growth and stress response. In this study, we reported that the knockout of OsPRP1 induced cold sensitivity in rice. Mutant plants were generated by CRISPR/Cas9 technology to investigate the role of OsPRP1 in cold stress and 26 mutant plants were obtained in T0 generation with the mutation rate of 85% including 15% bi-allelic, 53.3% homozygous, and 16.7% heterozygous and 16 T-DNA-free lines in T1 generation. The conserved amino acid sequence was changed and the expression level of OsPRP1 was reduced in mutant plants. The OsPRP1 mutant plants displayed more sensitivity to cold stress and showed low survival rate with decrease... More

关键词

CRISPR/Cas9; Cold Stress; Mutation; OsPRP1; Proline; Rice