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Phosphorylation and Alternative Splicing of 7B2 Reduce Prohormone Convertase 2 Activation.

Mol. Endocrinol.. 2015-05; 
Ramos-MolinaBruno,Lindberg
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Mutagenesis Services … plasmid was obtained from Addgene Mutagenesis reactions were carried out by Genscript using the pCEP4-r7B2 vector (27 kDa form) as a template and confirmed by sequencing of the entire insert The human 7B2 constructs … Get A Quote

摘要

FAM20C is a secretory kinase responsible for the phosphorylation of multiple secreted proteins in mammalian cells; it has been shown to phosphorylate serine residues within a variety of different bone proteins. In this work we demonstrate that FAM20C also phosphorylates threonines, specifically those within the N-terminal domain of the neuroendocrine chaperone 7B2. Analysis of the primary sequence of 7B2 revealed that three threonine residues in its N-terminal domain are located within FAM20C consensus motifs: Thr73, Thr99, and Thr111. The individual substitution of Thr73 and Thr111 residues by neutral alanines caused a marked decrease in the total phosphorylation of 7B2. Furthermore, the phosphomimetic... More

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