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Interleukin-2 Receptor β Thr-450 Phosphorylation Is a Positive Regulator for Receptor Complex Stability and Activation of Signaling Molecules.

J. Biol. Chem.. 2015; 
Ruiz-MedinaBlanca E,RossJeremy A,KirkenRobe
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Molecular Biology Tools … The amplification reac- tion was carried out in 25μl reaction volume consisting of 50 ng genomic DNA template, 1x PCR buffer (Biotools, B& M Labs, Madrid, Spain) with 2 mmol/L MgCl2, 04mmol/L of each primer (Genscript, Piscataway, NJ), 50mmol/L dNTPs (Biotools, B & M … Get A Quote

摘要

T, B, and natural killer cells are required for normal immune response and are regulated by cytokines such as IL-2. These cell signals are propagated following receptor-ligand engagement, controlling recruitment and activation of effector proteins. The IL-2 receptor β subunit (IL-2Rβ) serves in this capacity and is known to be phosphorylated. Tyrosine phosphorylation of the β chain has been studied extensively. However, the identification and putative regulatory roles for serine and threonine phosphorylation sites have yet to be fully characterized. Using LC-MS/MS and phosphospecific antibodies, a novel IL-2/IL-15 inducible IL-2Rβ phosphorylation site (Thr-450) was identified. IL-2 phosphokinetic ... More

关键词

JAK,STAT transcription factor,cell signaling,interleukin 2,lymphocyte,protein kinase,protein phosphatase,receptor phosphorylation,serine/threonine,signal transduc