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Flexible Linker Modulates Glycosaminoglycan Affinity of Decorin Binding Protein A.

Biochemistry. 2015; 
MorganAshli,SepuruKrishna Mohan,FengWei,RajarathnamKrishna,Wa
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ORF cDNA Clones/MolecularCloud … Expression and Purification of N40 DBPA Variants Open reading frame (ORF) of N40 DBPA was synthesized by GenScript Inc (Piscataway, NJ) The ORF for the wild-type (WT) mature DBPA strain N40 (residues 29–194) was … Get A Quote

摘要

Decorin binding protein A (DBPA) is a glycosaminoglycan (GAG)-binding adhesin found on the surface of the bacterium Borrelia burgdorferi (B. burgdorferi), the causative agent of Lyme disease. DBPA facilitates bacterial adherence to extracellular matrices of human tissues and is crucial during the early stage of the infection process. Interestingly, DBPA from different strains (B31, N40, and PBr) show significant differences in GAG affinities, but the structural basis for the differences is not clear. In this study, we show that GAG affinity of N40 DBPA is modulated in part by flexible segments that control access to the GAG binding site, such that shortening of the linker leads to higher GAG affin... More

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