| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | The pACYC-NHCT plasmid was constructed in a pACYCDuet-1 vector (P15A replicon, ChloramphenicolR, from Novagen) by subcloning a synthesized NcoI-BamHI gene fragment (GenScript, Piscataway, NJ, USA) carrying genes encoding NphT7 (acetoacetyl-CoA synthase from Streptomyces sp...The pET-AdhE2 plasmid was constructed in a pET-Duet vector (f1 origin, AmpicillinR, from Novagen) with adhE2 by subcloning synthesized gene encoding AdhE2 (aldehyde-alcohol dehydrogenase, from C. acetobutylicum ATCC 824, GenBank ID: Q9ANR5) from a pUC57 parent vector provided by GenScript into pET-Duet vector, using restriction sites NcoI and AvrII.from a pUC57 parent vector provided by GenScript into a pRSF-Duet1 vector using NcoI and AvrII restriction sites. | Get A Quote |
Propane (C3H8) is a volatile hydrocarbon with highly favourable physicochemical properties as a fuel， in addition to existing global markets and infrastructure for storage， distribution and utilization in a wide range of applications. Consequently， propane is an attractive target product in research aimed at developing new renewable alternatives to complement currently used petroleum-derived fuels. This study focuses on the construction and evaluation of alternative microbial biosynthetic pathways for the production of renewable propane. The new pathways utilize CoA intermediates that are derived from clostridial-like fermentative butanol pathways and are therefore distinct from the first microbial propan... More
