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Digital Microfluidics for Immunoprecipitation.

Anal. Chem.. 2016-11; 
SealeBrendon,LamCharis,RackusDarius G,ChamberlainM Dean,LiuChang,WheelerAar
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Biochemicals … Human serum albumin (HSA, molecular weight based on amino acid composition of 66 437 Da) solutions were prepared from lyophilized solids. Fluorescein-isothiocyanate labeled human serum albumin (FITC-HSA) was purchased from GenScript USA Inc. (Piscataway, NJ) … Get A Quote

摘要

Immunoprecipitation (IP) is a common method for isolating a targeted protein from a complex sample such as blood, serum, or cell lysate. In particular, IP is often used as the primary means of target purification for the analysis by mass spectrometry of novel biologically derived pharmaceuticals, with particular utility for the identification of molecules bound to a protein target. Unfortunately, IP is a labor-intensive technique, is difficult to perform in parallel, and has limited options for automation. Furthermore, the technique is typically limited to large sample volumes, making the application of IP cleanup to precious samples nearly impossible. In recognition of these challenges, we ... More

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