Products/Services Used | Details | Operation |
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Biochemicals> | … Drugs used and suppliers were: endothelin-1 (GenScript, Piscataway, NJ, USA); methoxamine hydrochloride (Sigma, St Louis, MO, USA); phenylephrine hydrochloride (Sigma); prazosin hydrochloride (Sigma); trovafloxacin mesylate (Tocris Bioscience, Bristol, UK) and U46619 … | Get A Quote |
Trovafloxacin, a fluroquinolone antibiotic, was recently found to be an inhibitor of pannexin-1 channels through which ATP is released as "find-me" signals in apoptotic Jurkat cells. Our interest in the role of pannexin-1 channels in α-adrenoceptor-mediated vasoconstriction led us to the novel finding reported here. Concentration-response curves to methoxamine and phenylephrine were competitively antagonised by trovafloxacin (1-30µM) with a pK of 5.54 and 5.32, respectively, in rat mesenteric small arteries isolated for myography. In comparison, prazosin (1-10nM) antagonised methoxamine concentration-response curves with a pK of 9.76. Trovafloxacin (1-30µM) had no effect on either the thromboxane m... More