Products/Services Used | Details | Operation |
---|---|---|
Mutagenesis Services> | … plasmid encoding DDK-tagged human CSNK1A1 was obtained from Origene (catalogue number RC217936, corresponding to NCBI reference sequence NP_001020276.1, splice form 1). Mutagenesis of Asp 136 to Asn (GAC—>AAC) was performed at GenScript USA, Inc | Get A Quote |
We describe the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1α (CSNK1A1) using activity-based proteomics. Matched normal and tumor colon samples were analyzed using an ATP acyl phosphate probe in a kinase-targeted LC-MS2 platform. An anomaly in the active-site peptide from CSNK1A1 was observed in a tumor sample that was consistent with an altered catalytic aspartic acid. Expression and analysis of the suspected mutant verified the presence of asparagine in the probe-labeled, active-site peptide for CSNK1A1. Genomic sequencing of the colon tumor samples confirmed the presence of a missense mutation in the catalytic aspartic acid of CSNK1A1 (GAC→AAC). To ... More