至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

An Engineered Hybrid Protein from Allergens Shows Hypoallergenicity.

Int J Mol Sci. 2019; 
MartínezDalgys,MuneraMarlon,CantilloJose Fernando,WortmannJudith,ZakzukJosefina,KellerWalter,CaraballoLuis,PuertaLeon
Products/Services Used Details Operation
Bacterial Expression System … generate images [55]. The synthesis of the nucleotide sequence coding for the designed protein was ordered from GenScript, (Piscataway, NJ, USA) with codon optimization for the efficient expression in E. coli. The sequence … Get A Quote

摘要

The house dust mite (HDM) is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that is based on recombinant proteins has been proposed for the safer and more efficient treatment of allergic diseases. The aim of this study was to design and obtain a hybrid protein (DPx4) containing antigenic regions of allergens Der p 1, Der p 2, Der p 7, and Der p 10 from this mite. DPx4 was produced in and its folding was determined by circular dichroism. Non-denaturing dot-blot, ELISA, basophil activation test, dot blot with monoclonal antibodies, ELISA inhibition, and cysteine protease activity assays were performed. Mice that were immunized with DPx4 were also analyzed. We f... More

关键词

IgE,allergen immunotherapy,allergy,hybrid protein,recombinant alle