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Bacterial Expression System> | … generate images [55]. The synthesis of the nucleotide sequence coding for the designed protein was ordered from GenScript, (Piscataway, NJ, USA) with codon optimization for the efficient expression in E. coli. The sequence … | Get A Quote |
The house dust mite (HDM) is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that is based on recombinant proteins has been proposed for the safer and more efficient treatment of allergic diseases. The aim of this study was to design and obtain a hybrid protein (DPx4) containing antigenic regions of allergens Der p 1, Der p 2, Der p 7, and Der p 10 from this mite. DPx4 was produced in and its folding was determined by circular dichroism. Non-denaturing dot-blot, ELISA, basophil activation test, dot blot with monoclonal antibodies, ELISA inhibition, and cysteine protease activity assays were performed. Mice that were immunized with DPx4 were also analyzed. We f... More