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Detection of canine Schistosoma japonicum infection using recombinant thioredoxin peroxidase-1 and tandem repeat proteins.

J. Vet. Med. Sci.. 2019; 
AngelesJose Ma M,GotoYasuyuki,KirinokiMasashi,LeonardoLydia R,MoendegKharleezelle J,YbañezAdrian P,RiveraPilarita T,VillacorteElena A,InoueNoboru,ChigusaYuichi,KawazuShin-Ic
Products/Services Used Details Operation
PCR Cloning and Subcloning … previously described. In brief, cloning of SjTPx-1 was done using S. japonicum Yamanashi strain adult worm cDNA while the 4 TRPs' nucleotides coding a partial tandem repeat domain were synthesized by GenScript USA Inc. (Piscataway, NJ, USA) … Get A Quote

摘要

Humans and dogs live very close together and share various pathogens causing zoonotic parasitoses like schistosomiasis. A previous population genetics study done for schistosomes in the Philippines suggested that there is a high transmission level of Schistosoma japonicum among humans and dogs proving that the latter are important reservoirs for this zoonotic parasite. A more sensitive and specific test detecting schistosome infection in dogs will therefore strengthen the zoonotic surveillance, which might help in the possible elimination of this ancient disease. In this study, recombinant thioredoxin peroxidase-1 (SjTPx-1) and tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, Sj7TR) previously tested on h... More

关键词

Schistosoma japonicum,diagnosis,recombinant antigen,veterinary public health,zoonotic schistosomi