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Androgen up-regulates vascular endothelial growth factor expression in prostate cancer cells via an Sp1 binding site.

Mol. Cancer. 2013; 
EisermannKurtis，BroderickCarly J，BazarovAnton，MoazamMustafa M，FraizerGa
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Monoclonal Antibody Services After transfer to a Polyvinylidene Fluoride (PVDF) membrane and blocking with 5% casein, blots were probed overnight at 4°C with polyclonal VEGF and AR antibodies (Santa Cruz), and monoclonal β-actin antibody (GenScript). Washed blots were then incubated for 1 hr in either HRP-conjugated anti – rabbit (GenScript) or anti – mouse (Santa Cruz) antibodies. Get A Quote

摘要

Vascular Endothelial Growth Factor (VEGF) is regulated by a number of different factors， but the mechanism(s) behind androgen-mediated regulation of VEGF in prostate cancer are poorly understood.

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