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Oligonucleotide array-in-well platform for detection and genotyping human adenoviruses by utilizing upconverting phosphor label technology.

Anal. Chem.. 2011; 
YlihärsiläMinna,ValtaTimo,KarpMaija,HattaraLiisa,HarjuEmilia,HölsäJorma,SavirantaPetri,WarisMatti,Soukka
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DNA Sequencing … increments. The resulting biotinylated amplicons were used unpurified in hybridizations or were purified (QuickClean, PCR Purification Kit, GenScript, USA) and eluted to a 30 μL volume of nuclease-free sterile water for sequencing … Get A Quote

摘要

We have developed a robust array-in-well test platform based on an oligonucleotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human i... More

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