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Chemical Protein Synthesis with the α-Ketoacid-Hydroxylamine Ligation.

Acc. Chem. Res.. 2017; 
BodeJeffr
Products/Services Used Details Operation
Biochemicals … NA Inc. (Morrisville, NC, USA). Human oxytocin was from Prospec Inc. Tuftsin was obtained from Bachem. Leu-enkephalin was obtained from GenScript Inc. All other materials and reagents were from Sigma-Aldrich. Example 1 … Get A Quote

摘要

The coupling of an α-ketoacid and a hydroxylamine (KAHA ligation) affords amide bonds under aqueous, acidic conditions without the need for protecting groups or coupling agents. Translating this finding into a general approach to chemical protein synthesis required the identification of methods to incorporate the key functional groups into unprotected peptide segments-ideally using well-established Fmoc solid-phase peptide synthesis protocols. A decade of effort has now led to robust, convenient methods for preparing peptides bearing free or masked C-terminal α-ketoacids and N-terminal hydroxylamines. The facile synthesis of the segments and the aqueous, acidic conditions of the KAHA ligation make it id... More

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