| Products/Services Used | Details | Operation |
|---|---|---|
| Nucleic Acid Purification & Analysis | The purified Mic1 particles were mixed with an equal volume of 23 electrophoresis loading buffer (62.5 mM Tris-HCl, 2% polyacrylamide, 10% glycerol, 2% 2-mercaptoethanol and 0.1% bromophenol blue, pH 6.8) at 95C for 10 min, and loaded onto a 4-12% gradient polyacrylamide gel (GenScript). P | Get A Quote |
Cyanobacteria are the most abundant photosynthetic microorganisms， the global distribution of which is mainly regulated by the corresponding cyanophages. A systematic screening of water samples in the Lake Chaohu enabled us to isolate a freshwater siphocyanophage that infects Microcystis wesenbergii， thus termed Mic1. Using cryoelectron microscopy， we solved the 3.5-? structure of Mic1 capsid. The major capsid protein gp40 of an HK97-like fold forms two types of capsomers， hexons and pentons. The capsomers interact with each other via the interweaved N-terminal arms of gp40 in addition to a tail-in-mouth joint along the three-fold symmetric axis， resulting in the assembly of capsid in a mortise-and-te... More
