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Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity.

Electrophoresis. 2017; 
Schwartz Kimberly,Bochkariov Dm
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis ERK2 Genscript A01194 PVDF HeLa rabbit/pAb 17.6 Get A Quote

摘要

Western blotting is a ubiquitous tool used in protein and molecular biology research, providing information about the presence, size, relative abundance, and state of a protein in a mixture. First, the proteins in a sample are separated by size using SDS-PAGE then transferred onto a membrane for detection with a set of primary and secondary antibodies. High-quality Western data requires high signal-to-noise ratios, which depend upon reduction of nonspecific antibody interactions. Blocking is a critical step in the Western blot method as it prevents the antibodies from binding nonspecifically to the membrane and irrelevant proteins. A solution of nonfat dry milk (NFDM) in physiological buffer is comm... More

关键词

Blocking solution,Immunoblotting,Nonfat dry milk,Protein research,Western