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IL-1β functionally attenuates ABCG2 and PDZK1 expression in HK-2 cells partially through NF-?B activation.

Cell Biol. Int.. 2019; 
LuXiaoyong,ChenMo,ShenJingfang,XuYujia,WuHuax
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DNA Sequencing Randomly picked 5 white colonies for plasmid isolation, and then DNA sequencing analysis, using T7 promoter primers, was performed with a 3730 Genetic Analyzer (GenScript, Nanjing, China). Get A Quote

摘要

Long-standing untreated hyperuricemia could lead to gout. Several recent studies have demonstrated a significant decrease of serum urate during acute gout attack, which is an aseptic inflammation process focusing on IL-1β. However, how IL-1β, by itself, alters the expression and the functional activity of urate transporters in renal tubular epithelial cells is still unclear. Herein, we revealed that IL-1β could attenuate the mRNA and protein levels of ABCG2, a major urate efflux pump, in HK-2 cells by real-time PCR and Western-blot assays. Moreover, using an ABCG2 specific inhibitor and a new sensitive and specific detection system, it was found that IL-1β also reduced the ABCG2 transporte... More

关键词

ABCG2,HK-2 cell lines,PDZK1,inflammation,u