至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The Interaction of Natural Selection and GC Skew May Drive the Fast Evolution of a Sand Rat Homeobox Gene.

Mol. Biol. Evol.. 2019; 
DaiYichen,HollandPeter
Products/Services Used Details Operation
Mutagenesis Services Materials and Methods Plasmid construction and in vitro mutagenesis Full coding sequences for mouse and sand rat Pdx1 genes were synthesized by GenScript, with alterations in sand rat codon usage to lower GC content (Supplementary Material). Get A Quote

摘要

Several processes can lead to strong GC skew in localized genomic regions. In most cases, GC skew should not affect conserved amino acids because natural selection will purge deleterious alleles. However, in the gerbil subfamily of rodents, several conserved genes have undergone radical alteration in association with strong GC skew. An extreme example concerns the highly conserved homeobox gene Pdx1, which is uniquely divergent and GC rich in the sand rat Psammomys obesus and close relatives. Here, we investigate the antagonistic interplay between very rare amino acid changes driven by GC skew and the force of natural selection. Using ectopic protein expression in cell culture, pulse-chase labeling... More

关键词

Pdx1,biased gene conversion,genome evolution,homeodomain,mutation,protein evolu