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Custom DNA/RNA Oligos> | cDNA synthesis was performed using 3 μg of DNase-treated RNA, 1 μl of M-MLV reverse transcriptase (200 U/μl, Promega, Wis- consin, USA), 4 μl of 5X reaction buffer (Promega, Wis- consin, USA), 10 mM dNTP Mix (GenScript, New Jersey, USA), 1 μl of 500 μg/mL Oligo (dt)12–18, 2 μl of 0. | Get A Quote |
The transmitting tissue of the style is the pathway for pollen tube growth to the ovules and has components that function in recognizing and discriminating appropriate pollen genotypes. In Nicotiana tabacum, the class III pistil extensin-like (PELPIII) arabinogalactan protein is essential for the inhibition of N. obtusifolia pollen tube growth. The transmitting tissue-specific (TTS) arabinogalactan protein amino acid sequence and expression pattern is similar to PELPIII, but it facilitates self-pollinated N. tabacum. The TTS and PELPIII arabinogalactan protein can be divided into the less conserved N-terminal (NTD) and the more conserved C-terminal (CTD) domains. This research tested whether the NTD is the ... More