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Glutamine Synthetase (GS): A Key Enzyme for Nitrogen Assimilation in The Macroalga Gracilariopsis lemaneiformis (Rhodophyta).

J. Phycol.. 2019; 
LiuXiaojuan,HuanZhongyan,ZhangQingfang,ZhongMingqi,ChenWeizhou,AslamMuhammad,Du
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摘要

This study aimed to address the importance of glutamine synthetase II (GSII) during nitrogen assimilation in macroalga Gracilariopsis lemaneiformis. The cDNA full-length sequence of the three glGSII genes was revealed to have the 5' m G cap, 5'-untranslated region, open reading frame (ORF), 3'-untranslated region, and a 3' poly (A) tail. The three glGSIIs were classified into plastid glGS2 and cytosolic glGS1-1 and glGS1-2, having conserved GSII domains but different cDNA sequences. The complicated 5' end flanking region indicates complex function of glGS genes. glGS1 genes were significantly up-regulated under the different NH : NO ratio (i.e., 40:10, 25:25, 10:40, and 0:50) except glGS2 whic... More

关键词

Gracilariopsis lemaneiformis ,full sequence,glutamine synthetase,nitrogen assimilation,transcriptional l