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Recombinant Antibody Services> | Quantification and removal of LPS in recombinant granulysin LPS content in recombinant granulysin preparations was tested using the Limulus amoebocyte lysate (LAL) test (ToxinSen- sor Chromogenic LAL Endotoxin Assay Kit; GenScript, USA). | Get A Quote |
9 kDa granulysin is a protein present in the granules of human CTL and NK cells, with cytolytic activity against microbes and tumors. Previous work from our group demonstrated that this granulysin isoform induced apoptosis on hematological tumor cells and on primary tumor cells from B-CLL patients. In the present work, recombinant 9 kDa granulysin was used as an anti-tumoral agent to study its effect on tumor development in athymic "nude" mice models bearing human breast adenocarcinoma MDA-MB-231 or multiple myeloma NCI-H929-derived xenografts. Granulysin prevented the development of detectable MDA-MB-231-derived tumors. In addition, recombinant granulysin was able to completely eradicate NCI-H929-de... More