| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Antibody Services | o r g / b y g u e s t o n O c t o b e r 4 , 2 0 1 6 4 Nucleic Acids Research, 2016 a recombinant fragment spanning amino acid positions 1301–1698 of nvGW182 in rabbit and guinea pig, respec- tively (GenScript, USA).... For Nematostella samples the following antibodies were used in Western blotting and IP: ␣CNOT9 (OriGene) 1:1000; ␣nvGW182 (GenScript, described above) 1:1000; ␣nvAGO1 (GenScript, described above) 1:1000. | Get A Quote |
Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates， arthropods， nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here， we dissect the function of GW182 protein in the cnidarian Nematostella， separated by 600 million years from other Metazoa. Using cultured human cells， we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs， thereby inhibiting translation and promoting mRNA decay. Further， similarly to bilaterians， GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Ar... More
