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Using the endogenous CRISPR-Cas system of to delete the photochemical reaction center core subunit gene.

Appl. Environ. Microbiol.. 2019; 
Baker Patricia L,Orf Gregory S,Kevershan Kimberly,Pyne Michael E,Bicer Taner,Redding Kev
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Gene Synthesis The construct was synthesized (GenScript) with the following sequence (SalI, BsaI, and BglII recognition sites are underlined, December 2019 Volume 85 Issue 23 e01644-19 aem. Get A Quote
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摘要

In , as in many Firmicutes, deleting genes by homologous recombination using standard techniques has been unsuccessful. The cells tend to integrate the introduced plasmid into the chromosome by a single recombination event, rather than perform the double recombination required to replace the targeted locus. Transformation with a vector containing only a homologous recombination template for replacement of the photochemical reaction center gene produced colonies with multiple genotypes, rather than a clean gene replacement.Bacterial CRISPR-Cas systems have become powerful biotechnological tools for genome editing across all domains of life. In this study, we report the genetic structure of the Type I-... More

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