Products/Services Used | Details | Operation |
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Recombinant Antibody Services> | Expression of purified IgG1 recombinant mAbs V(D)J gene fragments were de novo synthesized and cloned (GenScript) into the plasmids containing rhesus IgG1/IgK/IgL constant regions. | Get A Quote |
Development of an HIV vaccine is a global priority. A major roadblock to a vaccine is an inability to induce protective broadly neutralizing antibodies (bnAbs). HIV gp41 bnAbs have characteristics that predispose them to be controlled by tolerance. We used gp41 2F5 bnAb germline knock-in mice and macaques vaccinated with immunogens reactive with germline precursors to activate neutralizing antibodies. In germline knock-in mice, bnAb precursors were deleted, with remaining anergic B cells capable of being activated by germline-binding immunogens to make gp41-reactive immunoglobulin M (IgM). Immunized macaques made B cell clonal lineages targeted to the 2F5 bnAb epitope, but 2F5-like antibodies were either ... More