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Enhancement of interferon induction by ORF3 product of hepatitis E virus.

J. Virol.. 2014; 
Nan Yuchen,Ma Zexu,Wang Rong,Yu Ying,Kannan Harilakshmi,Fredericksen Brenda,Zhang Yan
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud ORF3 sequences from strains of HEV genotypes II (GenBank acces- sion number M74506) and IV (accession number AB074915) were syn- thesized (GenScript, Piscataway, NJ) and cloned into the XhoI and EcoRI sites of the VenusC1 vector as described previously (15). Get A Quote

摘要

Hepatitis E virus (HEV) causes both the endemic and epidemic spread of acute hepatitis in many parts of the world. HEV open reading frame 3 (ORF3) encodes a 13-kDa multifunctional protein (vp13) that is essential for HEV infection of animals. The exact role of vp13 in HEV infection remains unclear. In this study, vp13 was found to enhance interferon (IFN) production induced by poly(I · C), a synthetic analog of double-stranded RNA. Poly(I · C) treatment induced a higher level of IFN-β mRNA in HeLa cells stably expressing vp13 than in control cells. Using a luciferase reporter construct driven by the IFN-β promoter, we demonstrated that vp13 enhanced retinoic acid-inducible gene I (RIG-I)-dependent luc... More

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