Products/Services Used | Details | Operation |
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Recombinant Antibody Services> | Before use, LPS was removed from the recombinant protein samples on a polymyxin B-modified resin column from Genscript (Nanjing, China), and the endotoxin concentration of each sample was checked and shown to be <0. | Get A Quote |
Tumor-targeted cytokines are a new class of pharmaceutical anticancer agents often considered superior to the corresponding unconjugated cytokines for therapeutic purposes. We generated a new fusion protein, dsNKG2D-IL-15, in which double NKG2D extracellular domains were fused to IL-15, in Escherichia coli. This fusion protein promoted the activation, proliferation, and cytotoxicity of NK cells, and bound to NKG2D ligand-positive tumor cells. These tumor cells were also more susceptible to NK-cell attack when decorated with dsNKG2D-IL-15. The administration of mouse dsNKG2D-IL-15 protein in vivo significantly retarded the growth of transplanted colon cancers and prolonged the survival of tumor-beari... More