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ORF cDNA Clones/MolecularCloud> | Gene overexpression, gene KO and KD Human Nrp2 (Accession#AF022860), CD46 (Accession#NM_002389), TGFbRIII (Accession#XM_006710867) and THBD (Accession#AF495471) were ordered from GenEZ ORF database (Genscript) and cloned in pCDH-EF1-MCS (System Biosciences).... HAP-1 KO by CRISPR/Cas9 of TGFbRIII was generated using three short guide RNAs (sgRNAs ordered from Genscript and individually cloned into the lentiviral vector plentiCRISPRv2 (addgene plasmid # 52961) (Sanjana et al. | Get A Quote |
Characterizing cell surface receptors mediating viral infection is critical for understanding viral tropism and developing antiviral therapies. Nevertheless, due to challenges associated with detecting protein interactions on the cell surface, the host receptors of many human pathogens remain unknown. Here, we build a library consisting of most single transmembrane human receptors and implement a workflow for unbiased and high-sensitivity detection of receptor-ligand interactions. We apply this technology to elucidate the long-sought receptor of human cytomegalovirus (HCMV), the leading viral cause of congenital birth defects. We identify neuropilin-2 (Nrp2) as the receptor for HCMV-pentamer infection i... More