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A python script to design site-directed mutagenesis primers

Protein Sci.. 2019; 
Bi C1,2, Huang X1,2, Tang D3, Shi Y2, Zhou L4, Hu Y5, Chen X1,2, Qi S3, Lin S1,4.
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摘要

Primer design is essential to conduct whole plasmid site-directed mutagenesis for protein study. Traditionally, primers of mutagenesis are designed manually that is time-consuming and fallible. Here, we present a Python script for searching primers by presetting parameters of nucleotide composition and percentage of guanine-cytosine (GC content). The running results showed that the script is able to search primers with mutations of target residue automatically. This script may facilitate primer design for whole plasmid site-directed mutagenesis and aid protein mutant construction.,© 2019 The Protein Society.

关键词

primer design; python script; site-directed mutagenesis