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P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes.

Methods Mol Biol. 2020;

Bertin E1, Martínez A1, Boué-Grabot E2.

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Antibody Modification and Purification Services	… Representative immunoblots of total and surface biotinylated proteins from oocytes expressing<br> rat P2X2-HIS or mouse P2X4 wild-type using anti-His (1:1500, <b>Genscript</b>) or anti-P2X4 (1:1000,<br> Alomone) primary antibodies respectively and horseradish peroxidase (HRP …	Get A Quote

摘要

Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.