Two potential fish glycerol-3-phosphate phosphatases.

Winter-acclimated rainbow smelt (Osmerus mordax Mitchill) produce high levels of glycerol as an antifreeze. A common pathway to glycerol involves the enzyme glycerol-3-phosphate phosphatase (GPP)， but no GPP has yet been identified in fish or any other animal. Here， two phosphatases assembled from existing EST libraries (from winter-acclimated smelt and cold-acclimated smelt hepatocytes) were found to resemble a glycerol-associated phosphatase from a glycerol-producing alga， Dunaliella salina， and a recently discovered GPP from a bacterium， Mycobacterium tuberculosis. Recombinant proteins were generated and were found to have GPP activity on the order of a few μMol Pi/mg enzyme/min. The two enzymes have acidic pH optima (~5.5) similar to that previously determined for GPP activity in liver tissue， with about 1/3 of their peak activities at neutral pH. The two enzymes appear to account for the GPP activity of smelt liver， but due to their reduced activities at neutral pH， their contributions to glycerol production in vivo remain unclear. Similar enzymes may be active in a glycerol-producing insect， Dendroctonus ponderosae.