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Peptide Synthesis> | … For antibody blocking experiments, control peptides and peptides encoding the immunogen for the KAT6B antibody (GenScript) were incubated overnight at 4 °C with α-KAT6B before staining as above Coverslips were mounted on slides with Gold Antifade with DAPI … | Get A Quote |
Aged hematopoietic stem cells (HSCs) undergo biased lineage priming and differentiation toward production of myeloid cells. A comprehensive understanding of gene regulatory mechanisms causing HSC aging is needed to devise new strategies to sustainably improve immune function in aged individuals. Here, a focused shRNA screen of epigenetic factors reveals that the histone acetyltransferase Kat6b regulates myeloid cell production from hematopoietic progenitor cells. Within the stem and progenitor cell compartment, Kat6b is highly expressed in long-term (LT)-HSCs and is significantly decreased with aging at the transcript and protein levels. Knockdown of Kat6b in young LT-HSCs causes skewed production of myeloid ce... More