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AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint.

Cell Cycle. 2017; 
López-Soop G,, Rønningen T,, Rogala A, Richartz N, Blomhoff HK, Thiede B, Collas P,, Küntziger T.
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Bacterial Expression System coli biotin ligase (hBirAR118G, BirA*, 21) and flanking Kozak and PolyA sites was synthetized (Genscript). Get A Quote

摘要

Faithful chromosome segregation during mitosis relies on a proofreading mechanism that monitors proper kinetochore-microtubule attachments. The spindle assembly checkpoint (SAC) is based on the concerted action of numerous components that maintain a repressive signal inhibiting transition into anaphase until all chromosomes are attached. Here we show that A-Kinase Anchoring Protein 95 (AKAP95) is necessary for proper SAC function. AKAP95-depleted HeLa cells show micronuclei formed from lagging chromosomes at mitosis. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKA... More

关键词

mitotic arrest deficient; translocated in promoter region