Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | The synthetic oligonucleotides with the following seqences were purchased from Nanjing GenScript Biotech Corp: hDNA: 5 ’-AAGGGTTGGGCGGGATGGGTTACCTCAGTGCTTATT CGAAACCCA-3 ’, (5' Amino Modified), complementary matched tDNA (CMM): TCGAATAAGCACTGAGGT-3 ’, double-base mismatched DNA ( DMM): TCGAACAAGCACCGAGGT-3 ’, four-base mismatched DNA (FM M): TCGCATACGCATTGAGTT-3 ’, non-complementary matched DN A (NCMM): 5 ’- 5 ’- 5 ’- 5 ’- ATCCTATTAGTTCATATC-3 ’. | Get A Quote |
Photoelectrochemical (PEC) DNA bioanalysis has been drawing more attention in recent years due to the advantages of PEC technique and the vital importance of DNA biomolecules. DNAzymes are unique catalytic nucleic acid molecules that are capable of catalyzing specific biochemical reactions. Using the target-binding-induced conformation change of hairpin DNA probe to hemin/G-quadruplex-based DNAzyme and a plasmonic Au@Ag nanoparticles (NPs)/TiO2 nanorods (NRs)/fluorine-doped tin oxide (FTO) heterostructured photoelectrode, this work reported a novel and sensitive PEC DNA analysis on the basis of a DNAzyme-stimulated biocatalytic precipitation (BCP) strategy. In such a design, the BCP-induced decrease of plasmoni... More