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The methyltransferase Suv39h1 links the SUMO pathway to HP1α marking at pericentric heterochromatin.

Nat Commun. 2016; 
Maison C,, Bailly D,, Quivy JP,, Almouzni G,.
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Mutagenesis Services The point mutations in the Ubc9 binding domain of Suv39h1 were generated from Myc-Suv39h1, His-Suv39h1-1-167 and TALE-Suv39h1-1-167-HA by site directed mutagenesis (GenScript). Get A Quote

摘要

The trimethylation of histone H3 on lysine 9 (H3K9me3) - a mark recognized by HP1 that depends on the Suv39h lysine methyltransferases (KMTs) - has provided a basis for the reader/writer model to explain HP1 accumulation at pericentric heterochromatin in mammals. Here, we identify the Suv39h1 paralog, as a unique enhancer of HP1α sumoylation both in vitro and in vivo. The region responsible for promoting HP1α sumoylation (aa1-167) is distinct from the KMT catalytic domain and mediates binding to Ubc9. Tethering the 1-167 domain of Suv39h1 to pericentric heterochromatin, but not mutants unable to bind Ubc9, accelerates the de novo targeting of HP1α to these domains. Our results establish an unexpected feature... More

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