| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | Secondary antibodies included: goat anti-rab- bit (A00098, 1:2,000 dilution; Genscript), goat anti-mouse (A00160, 1:2,000 dilution; Genscript), Alexa Fluor 555 donkey anti–rabbit IgG (1:300; Invitrogen), Alexa Fluor 488 donkey anti–mouse IgG (1:300; In- vitrogen), Alexa Fluor 488 goat anti–mouse IgG (1:300, Jackson Immu- noResearch Laboratories), Alexa Fluor 555 goat anti–mouse IgG (1:300; Invitrogen), Alexa Fluor 633 goat anti–mouse IgG (1:300; Invitrogen), Alexa Fluor 633 goat anti–mouse IgG (1:300; Invitrogen), and Alexa Fluor 633 goat anti–rabbit IgG (1:300; Invitrogen). | Get A Quote |
Neuroligin (NLG) 1 is important for synapse development and function, but the underlying mechanisms remain unclear. It is known that at least some aspects of NLG1 function are independent of the presynaptic neurexin, suggesting that the C-terminal domain (CTD) of NLG1 may be sufficient for synaptic regulation. In addition, NLG1 is subjected to activity-dependent proteolytic cleavage, generating a cytosolic CTD fragment, but the significance of this process remains unknown. In this study, we show that the CTD of NLG1 is sufficient to (a) enhance spine and synapse number, (b) modulate synaptic plasticity, and (c) exert these effects via its interaction with spine-associated Rap guanosine triphosphatase-activating... More
