| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | C for 2 minutes, 0 0 0 0 Construction of reporter constructs and infectious proviral genomes Generation of reporter construct containing the deletion identified in donor 436000 (p436-5LTRdel) A 2238 bp DNA fragment containing the donor 436000 5 LTR and gag sequence within NL4-3-DGPE-GFP BstZ171 to SwaI sites (see Figure S6) was synthesized by GenScript with flanking 5 SalI and 3 XbaI restriction sites and cloned by GenScript into the multiple cloning cassette of pUC18, the same vector as the parental viral plasmid for NL4-3-DGPE-GFP. | Get A Quote |
Long-lived reservoirs of persistent HIV are a major barrier to a cure. CD4+ hematopoietic stem and progenitor cells (HSPCs) have the capacity for lifelong survival, self-renewal, and the generation of daughter cells. Recent evidence shows that they are also susceptible to HIV infection in vitro and in vivo. Whether HSPCs harbor infectious virus or contribute to plasma virus (PV) is unknown. Here, we provide strong evidence that clusters of identical proviruses from HSPCs and their likely progeny often match residual PV. A higher proportion of these sequences match residual PV than proviral genomes from bone marrow and peripheral blood mononuclear cells that are observed only once. Furthermore, an analysis of ... More
