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Identification and analysis of mouse non-coding RNA using transcriptome data.

Sci China Life Sci. 2016; 
Zhao Y,, Liu W,, Zeng J,, Liu S,, Tan X, Aljohi H, Hu S.
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Molecular Biology Tools For amplifying the lincRNA transcripts, primer sets were designed by web-based tool GenScript (https://www....genscript. Get A Quote

摘要

Transcripts are expressed spatially and temporally and they are very complicated, precise and specific; however, most studies are focused on protein-coding related genes. Recently, massively parallel cDNA sequencing (RNA-seq) has emerged to be a new and promising tool for transcriptome research, and numbers of non-coding RNAs, especially lincRNAs, have been widely identified and well characterized as important regulators of diverse biological processes. In this study, we used ultra-deep RNA-seq data from 15 mouse tissues to study the diversity and dynamic of non-coding RNAs in mouse. Using our own criteria, we identified totally 16,249 non-coding genes (21,569 non-coding RNAs) in mouse. We annotated these non-c... More

关键词

RNA-seq; lincRNA; mouse; non-coding RNA; transcriptome